Device

Part:BBa_K598002

Designed by: Yan GONG, Shuobin CHEN, Yunpeng LIU, Xiaowei YAN   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 16:14, 27 September 2011 by Registry (Talk | contribs)

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Bistable Switch Mutant 68

RFP+RBS_SDA-C1+OR21 double directed promoter+SDcro CI434+eGFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2699


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//plasmidbackbone/copynumber/high
Parameters
chassisE.coli DH5α
device_typeLogic circuit
emission509nm for eGFP and 610 for mRFP
excitation470nm for eGFP and 580nm for mRFP
n/aBistable Switch Mutant 68
resistanceChloramphenicol