Device
Part:BBa_K598014
Designed by: Xiaowei YAN, Tong MU Group: iGEM11_Peking_R (2011-09-27)
pBAD+HH+CdIntron+E0040+b0015
This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1408
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//plasmidbackbone/copynumber/high
//direction/forward
//plasmidbackbone/copynumber/high
emission | 509nm |
excitation | 470nm |
n/a | pBAD+HH+CdIntron+E0040+b0015 |
resistance | chloramphenicol |