Regulatory
pRM

Part:BBa_K566001:Design

Designed by: Daniel Rodriguez, Eduardo Almeyda, Porfirio Quintero   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 16:05, 27 September 2011 by Quintero (Talk | contribs) (→‎References)

pRM promoter from Lambda


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Analysis of illegal restriction sites was performed, but it does not have them originally.


Source

It comes directly from genomic DNA of Lambda phage. It was extracted by PCR as recommended by the Registry.

References

1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.

2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154.