Device

Part:BBa_K598013

Designed by: Xiaowei YAN, Tong MU   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 15:59, 27 September 2011 by Registry (Talk | contribs)

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pBAD+theoHH+CdIntron+E0040+B0015

This a GFP reporter regulated by an allosteric intron responsive to theophylline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1433


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//plasmidbackbone/copynumber/high
//regulation/positive
Parameters
emission509nm
excitation470nm
ligandstheophylline
n/apBAD+theoHH+CdIntron+E0040+B0015
resistancechloramphenicol