Device

Part:BBa_K598010

Designed by: Siyang HAO, Zhenrun ZHANG   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 15:26, 27 September 2011 by Registry (Talk | contribs)

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pBAD+Theophylline Responsive Riboswitch 1G1 with Engineered RBS+E0040+B0015

It is a theophylline responsive riboswitch which contains a designed RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 868


[edit]
Categories
Parameters
chassisE. coli DH5α
ligandstheophylline
n/apBAD+Theophylline Responsive Riboswitch 1G1 with Engineered RBS+E0040+B0015
rbsrobust
resistanceChloramphenicol