Device
Part:BBa_K598009
Designed by: Siyang HAO, Zhenrun ZHANG Group: iGEM11_Peking_R (2011-09-27)
pBAD+Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015
It is a theophylline responsive riboswitch containing an original RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 869
[edit]
Categories
Parameters
chassis | E. coli DH5α |
ligands | theophylline |
n/a | pBAD+Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015 |
rbs | medium |
resistance | Chloramphenicol |