DNA

Part:BBa_K524000:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST   (2011-09-15)
Revision as of 13:38, 27 September 2011 by Chshek (Talk | contribs) (→‎Source)

Heat sensitive origin of replication (oriR101 & repA101-ts)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1709
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To make this part compatible with Biobrick standard assembly protocols, overlapping PCR was used to mutate a SpeI cut site originally present inside the construct to render it nonfunctional.


Source

From the plasmid pKD46 maintained inside the E.coli strain BW25113, courtesy of E.coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Center.

References