Generator
Part:BBa_K539431:Design
Designed by: Shu-Han Chang Group: iGEM11_NCTU_Formosa (2011-09-27)
promoter (lacI regulated)+RNA thermometer+vioD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1194
Illegal SapI.rc site found at 1269
Design Notes
Built in Plac promoter(Part:BBa_R0010), followed by 37’celcius regulator RBS (Part:BBa_K115002) and vioD(Part:BBa_K539413).
Source
The RNA thermometer comes from 2008 iGEM TUDelft and the vioD is cloned from 2010 iGEM Slovenia.