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Generator

Part:BBa_K539431

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)
Revision as of 09:43, 27 September 2011 by Zcc (Talk | contribs)

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promoter (lacI regulated)+RNA thermometer+vioD

Built in Plac promoter(Part:BBa_R0010), followed by 37’celcius regulator RBS (Part:BBa_K115002) and vioD(Part:BBa_K539413). It catalyze protodeoxyviolaceinic acid(PVA) into protoviolaceinic acid when the temperature hit 37’C or higher.It can be added any part you want to improvise its function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1194
    Illegal SapI.rc site found at 1269


[edit]
Categories
Parameters
n/apromoter (lacI regulated)+RNA thermometer+vioD