Regulatory
PalcA

Part:BBa_K678001:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-02)
Revision as of 08:04, 27 September 2011 by Jaide (Talk | contribs)

PalcA, inducible Aspergillus nidulans promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.

DTU-Denmark-2 2011


This part can easily be fused with other parts adapted to the Plug 'n' Play assembly standard as shown below.


DTU-Denmark-2 2011


This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug 'n' Play plasmids pJEJAM10, pJEJAM11, pJEJAM19, andpJEJAM20


Source

Aspergillus nidulans genomic DNA

References

Creaser, E.H., Porter, R.L., Britt, K.A., Pateman, J.A. and, Doy, C.H.: Purification and preliminary characterization of alcohol dehydrogenase from Aspergillus nidulans. Biochem. J. (1985) 225: 449-454.