Regulatory

Part:BBa_K619889:Design

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-26)
Revision as of 05:35, 27 September 2011 by Jgrose (Talk | contribs) (→‎Source)

prfA-UTR, RNA thermosensor from Listeria monocytogenes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.

References