Cell

Part:BBa_K615003:Design

Designed by: Naomi Genuth   Group: iGEM11_Harvard   (2011-09-26)
Revision as of 03:46, 27 September 2011 by Ngenuth (Talk | contribs) (→‎Design Notes)

E. coli strain for TolC one-hybrid selection system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 845
    Illegal BglII site found at 965
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
    Illegal NgoMIV site found at 181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 121
    Illegal SapI site found at 331


Design Notes

rpoZ knocked out using lambda red

Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC

Source

Based off EcNR2 selection strain (see Wang et al, 2009)

References