RNA

Part:BBa_K581006

Designed by: Qin Xiao   Group: iGEM11_Peking_Signaling   (2011-09-26)
Revision as of 03:32, 27 September 2011 by Registry (Talk | contribs)

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Pc+ptsG(wt)-GFP (ptsG(wt) 5' UTR fused with gfp)

This is the conjugate part of the small RNA regulator sgrS_wt.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790


[edit]
Categories
//chassis/prokaryote/ecoli
transcriptional
Parameters
biology5' regulatory region of ptsG(wt) is the target of sRNA SgrS(wt)
chassisE. coli DH5α
n/aPc+ptsG(wt)-GFP (ptsG(wt) 5' UTR fused with gfp)