RNA

Part:BBa_K581001

Designed by: Chen Yiwei   Group: iGEM11_Peking_Signaling   (2011-09-26)
Revision as of 03:21, 27 September 2011 by Registry (Talk | contribs)

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ptsG1-GFP (ptsG1 5' UTR fused with gfp)

This is the conjugate part of the small RNA sgrS1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 747


[edit]
Categories
//chassis/prokaryote/ecoli
transcriptional
Parameters
biology5' regulatory region of ptsG1 is the target of sRNA SgrS1
chassisE. coli DH5α
genotypeC85G mutant of ptsG(wt)
n/aptsG1 (conjugate part of SgrS1 in comparator)