Coding

Part:BBa_K615004

Designed by: Naomi Genuth   Group: iGEM11_Harvard   (2011-09-26)
Revision as of 00:48, 27 September 2011 by Registry (Talk | contribs)

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Zif268 binding site-His3-URA3 construct for one-hybrid selection

This is one of the two components necessary for a metabolic His3-URA3 selection system (the other being a zinc finger fused to the omega subunit of RNA polymerase).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 15
    Illegal BglII site found at 528
    Illegal BglII site found at 588
    Illegal BamHI site found at 818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1508
    Illegal SapI.rc site found at 1355


[edit]
Categories
Parameters
None