Cell

Part:BBa_K615003:Design

Designed by: Naomi Genuth   Group: iGEM11_Harvard   (2011-09-26)
Revision as of 21:20, 26 September 2011 by Registry (Talk | contribs)

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E. coli strain for TolC one-hybrid selection system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 845
    Illegal BglII site found at 965
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 632
    Illegal PstI site found at 952
    Illegal NgoMIV site found at 181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 121
    Illegal SapI site found at 331


Design Notes

rpoZ knocked out using lambda red Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC


Source

Based off EcNR2 selection strain (see Wang et al, 2009)

References