Composite

Part:BBa_K634007:Experience

Designed by: Eric Walters   Group: iGEM11_Wisconsin-Madison   (2011-09-23)
Revision as of 20:52, 26 September 2011 by Ewalters (Talk | contribs) (→‎Applications of BBa_K634007)

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Please enter how you used this part and how it worked out.

Applications of BBa_K634007

When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways:

Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence)

Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin.

Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose

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UNIQ75eaca076648feff-partinfo-00000000-QINU UNIQ75eaca076648feff-partinfo-00000001-QINU