Cell

Part:BBa_K615002

Designed by: Naomi Genuth   Group: iGEM11_Harvard   (2011-09-26)
Revision as of 19:34, 26 September 2011 by Ngenuth (Talk | contribs)

EcNR2 E. coli strain

The EcNR2 strain is a modified E. coli strain which has been designed for easy genome manipulation. It contains the lambda red machinery and the MutS knockout needed for efficient lambda red and multiplex automated genome engineering (MAGE).

See below for a summary of strain features and techniques:

HARVEcnr2summary.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologygrow only at 30⁰C
genotypeMG1655 with λ-prophage::bioA/bioB and cmR::mutS
resistancechloramphenicol, ampicillin