Coding
cI opt

Part:BBa_K566012:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 18:58, 26 September 2011 by Registry (Talk | contribs)

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cI repressor from Lambda phage optimized for E. coli cI (+LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites for classic BioBricks was performed, they were detected and eliminated.


Source

Sequence was obtained form BioBrick BBa_C0052, it was optimized changing for preferential codons as possible and then synthesized.

References