Coding
PenI inv

Part:BBa_K566010

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 18:28, 26 September 2011 by Jimmybly (Talk | contribs)

PenI repressor optimized for E. coli (inverted sequence)

penI repressor from Bacillus licheniformis (+LVA) with codon optimization for improving expression into E. coli.

This protein represents a negative repressor for pPenI promoter (BBa_R0074).

Includes LVA tag.

Sequence was inverted for simplifying DNA synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None