Intermediate

Part:BBa_K526000:Design

Designed by: Sung Kuk Lee   Group: iGEM11_UNIST_Korea   (2011-09-26)
Revision as of 07:34, 26 September 2011 by Skleelab (Talk | contribs) (→‎Design Notes)

Pompc promoter coding fimE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 750
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no special design consideration. We can amplify the Pompc and fimE from E. coli genomic DNA and assembly them using Gibson's assembly.

tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagtcacacaggacggccgggtgagtaaacgtcgttatcttaccggtaaagaagttcaggccatgatgcaggcggtttgttacggggcaacgggagccagagattattgtcttattctgttggcatatcggcatgggatgcgtattagtgaactgcttgatctgcattatcaggaccttgaccttaatgaaggtagaataaatattcgccgactgaagaacggattttctaccgttcacccgttacgttttgatgagcgtgaagccgtggaacgctggacccaggaacgtgctaactggaaaggcgctgaccggactgacgctatatttatttctcgccgcgggagtcggctttctcgccagcaggcctatcgcattattcgcgatgccggtattgaagctggaaccgtaacgcagactcatcctcatatgttaaggcatgcttgcggttatgaattggcggagcgtggtgcagatactcgtttaattcaggattatctcgggcatcgaaatattcgccatactgtgcgttataccgccagtaatgctgctcgttttgccggattatgggaaagaaataatctcataaacgaaaaattaaaaagagaagaggtttga

Source

Both fimE and Pompc was amplified from E. coli genomic DNA.

Ham, T.S., Lee, S.K., Keasling, J.D. and Arkin, A.P. A tightly regulated inducible expression system utilizing the fim inversion recombination switch. Biotechnology and Bioengineering 94 (2006), pp. 1-4.


References