Composite

Part:BBa_K575030

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)
Revision as of 01:10, 26 September 2011 by Mvs678 (Talk | contribs)

LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP, Constitutive Promoter + RBS (B0030) + LasR

Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 904
    Illegal NheI site found at 927
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal AgeI site found at 738
    Illegal AgeI site found at 850
    Illegal AgeI site found at 1480
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None