Regulatory

Part:BBa_K511000

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)
Revision as of 22:20, 25 September 2011 by Registry (Talk | contribs)

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Hef1a-LacOid Promoter MammoBlock

This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor.

As shown in Figures 1 and 2 below, cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 292
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BamHI site found at 255
    Illegal XhoI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal NgoMIV site found at 804
    Illegal AgeI site found at 80
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHef1a-LacOid Promoter MammoBlock