Coding
Part:BBa_K642001
Designed by: Wilson Lam, Matthew Orton Group: iGEM11_uOttawa (2011-09-22)
LacI repressor tagged with yBFP
This is the LacI repressor from E.coli tagged with yeast codon optimized BFP. LacI will bind to the the operator LacO and is inhibited in the presence of IPTG. (1) BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.
References
(1)Scrable, H. & Stambrook, P.J. (1997). "Activation of the lac repressor in the transgenic mouse." Genetics 147: 297–304.
(2)Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1084
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | LacI repressor tagged with yBFP |