Coding

Part:BBa_K642000

Designed by: Wilson Lam, Matthew Orton   Group: iGEM11_uOttawa   (2011-09-22)
Revision as of 19:42, 25 September 2011 by Morto077 (Talk | contribs)

TetR repressor tagged with yBFP

This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein converted from TagRFP(1). It has an excitation peak of 399 nm and an emission peak of 465 nm(1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTetR repressor tagged with yBFP