Part:BBa_K411001:Experience

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User Reviews

UNIQa7114ce9509326b0-partinfo-00000000-QINU

No review score entered. NYMU-Taipei iGEM10

[http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch Experimental Data] using Part:BBa_K411003 to test this part. Works. Downstream translation strength is proportional to the amount of theophylline.

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BBa_K411001 Wits-CSIR_SA iGEM2011

Team NYMU Taipei assumed that a created theophylline riboswitch (Gallivan lab clone 8.1) biobrick could be fused to a GFP coding region by standard biobrick assembly techniques. While it is possible to do this, we feel that the resulting 6 nt scar sequence insertion increases the distance between the RBS and the ATG start codon, a result which is likely to cause a decrease in riboswitch efficiency (see team Taipei switch below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined). In fact, Taipei 2010 did show data which showed only a 2.5 fold increase in gene activation at approx. 4 mM theophylline. Compared to the 36-fold activation at 1mM theophylline by Gallivan lab, this seems too low! We therefore feel that this riboswitch is not behaving efficiently. Part:BBa_K411001 Part:BBa_K411003 - Team NYMU-Taipei iGEM2010

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