Composite
Part:BBa_K539142:Design
Designed by: Yang, Yi-Pei Group: iGEM11_NCTU_Formosa (2011-09-19)
Revision as of 05:02, 25 September 2011 by Bettyyang (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K539142 short</partinfo> <partinfo>BBa_K539142 SequenceAndFeatures</partinfo> ===Design Notes=== In our experiment, the intensity of RNA thermometer are °C, 42...)
crtEBI+RNA thermometer+crtY+terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1974
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1510
Illegal NgoMIV site found at 1640
Illegal NgoMIV site found at 4611
Illegal AgeI site found at 725 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our experiment, the intensity of RNA thermometer are °C, 42°C, 32°C, 27°C. With the help of RNA thermometer, we can control the color of pigment by different temperatures. When the temperature is lower than 37°C, the pigment is red because the translation of crtEBI. When the temperature is higher than 37°C, the pigment is orange because of the translation of crtY that convert lycopene to orange beta-carotene.
Source
Other parts come from parts-registry.