Part:BBa_K615001

Zinc finger-omega subunit fusion protein

This protein is designed for one-hybrid selection systems testing for zinc finger binding. In a strain such as BBa_K615000, where the omega subunit of RNA polymerase has been knocked out, zinc finger binding can be used to control expression of genes necessary for cell survival. Properly bound zinc fingers recruit RNA polymerase to transcribe genes such as His3, allowing the bacteria to grow in incomplete media, while improperly bound zinc fingers will not activate His3 and thus will not survive.

This array is composed of three zinc fingers: Fingers 2 (F2) and 3 (F3) are known from CoDA studies, while F1 is a novel zinc finger designed to bind to the sequence TGG, together creating a zinc finger binding site of 5'-GTG GGA TGG-3'. The part is also designed to allow F1 to be easily replaced by other zinc fingers to allow testing of many different fingers' abilities to bind to the TGG codon.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 270
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 309
  
• 1000
  COMPATIBLE WITH RFC[1000]