Coding

Part:BBa_K530000

Designed by: Daniel Wolozny   Group: iGEM11_Johns_Hopkins   (2011-08-01)
Revision as of 23:11, 24 September 2011 by Danielw9 (Talk | contribs)

CRTYB

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is a combination, it is the full Phytoene Synthase enzyme spliced with a Lycopene B-Cyclase enzymatic domain. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1068
    Illegal BsaI.rc site found at 1399



Plasmid Map

CrtYB Plasmid Map.png

[edit]
Categories
Parameters
None