Plasmid

Part:BBa_K530038:Design

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)
Revision as of 22:33, 24 September 2011 by Registry (Talk | contribs)

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pRSBB425 LEU2 2Micron Episomal Yeast Shuttle Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1116
    Illegal XbaI site found at 6309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1116
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1116
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1116
    Illegal XbaI site found at 6309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1116
    Illegal XbaI site found at 6309
    Illegal NgoMIV site found at 2799
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4479
    Illegal SapI site found at 3396


Design Notes

QuickChange PCR


Source

pRS 425

References