Plasmid
Part:BBa_K530037:Design
Designed by: Daniel Bibl Group: iGEM11_Johns_Hopkins (2011-09-24)
pRSBB424 TRP1 2Micron Episomal Yeast Shuttle Vector
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 551
Illegal XbaI site found at 5076 - 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 551
Illegal XbaI site found at 5076 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 551
Illegal XbaI site found at 5076
Illegal NgoMIV site found at 1566 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3246
Illegal SapI site found at 2163
Design Notes
QuickChange PCR
Source
pRS 424