Cell
Part:BBa_K615000
Designed by: Naomi Genuth Group: iGEM11_Harvard (2011-09-21)
E. coli strain for His3-URA3 one-hybrid selection system
This strain is designed to test zinc finger binding using a one-hybrid metabolic selection system. The endogenous genes HisB and PyrF have been knocked out and replaced with the yeast versions His3 and URA3 under the control of a Zif268 binding site. The gene coding for the omega subunit of RNA polymerase (rpoZ) was knocked out by replacing it with a Zeocin cassette, and the strain is designed to be used in conjunction with BBa_K615001 (zinc finger-omega subunit expression construct).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal PstI site found at 1762 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal PstI site found at 1762 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 845
Illegal BglII site found at 965
Illegal BglII site found at 1478
Illegal BglII site found at 1538
Illegal BamHI site found at 1768 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal PstI site found at 1762 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal PstI site found at 1762
Illegal NgoMIV site found at 181 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2458
Illegal SapI site found at 121
Illegal SapI site found at 331
Illegal SapI.rc site found at 2305
Functional Parameters
| biology | grow only at 30⁰C |
| genotype | l-prophage::bioA/bioB cmR::mutS ΔHisBΔPyrFΔrpoZ + His3+URA3 |
| resistance | Ampicillin, Chloramphenicol, Kanamycin, Zeocin |
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Categories
Parameters
| biology | grow only at 30⁰C |
| genotype | l-prophage::bioA/bioB cmR::mutS ΔHisBΔPyrFΔrpoZ + His3+URA3 |
| resistance | Ampicillin, Chloramphenicol, Kanamycin, Zeocin |