Coding
AAR-ADC

Part:BBa_K590051

Designed by: Austin Moon, Casey Ager, Ingrid Swanson, Seth Sagulo   Group: iGEM11_Washington   (2011-09-15)
Revision as of 21:22, 24 September 2011 by Anjuhye (Talk | contribs) (→‎Usage and Biology)

Acyl-ACP Reductase to Aldehyde Decarbonylase (AAR-ADC) Fusion

This part encodes a direct gene fusion product of the two enzymes, Acyl-ACP Reductase(AAR) and Aldehyde Decarbonylase (ADC), in a linear configuration with a glycine-serine linker for co-localization optimization of ADC alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

Usage and Biology

We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together.

Direct fusion of two proteins using a glycine-serine linker.
Contrary to what was expected, our GCMS data demonstrated that direct fusion has a negative effect on alkane production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None