Measurement

Part:BBa_K516334:Design

Designed by: Federica Sampietro, Susanna Zucca, Tommaso Goggia, Paolo Magni   Group: iGEM11_UNIPV-Pavia   (2011-09-08)
Revision as of 15:49, 24 September 2011 by Fedesampi (Talk | contribs)

mRFP producer controlled by 3OC6-HSL with RBS B0034


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1550
    Illegal AgeI site found at 1662
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 912


Design Notes

The BioBrick parts were selected to reduce the internal homology, that could be cause of recombination or mutation events (Sleight SC et al. 2010).
The choise of Transcriptional terminator was done purposely: in fact the alligniment of the single terminator BBa_B1006 and the double terminator BBa_B0015 does not show a significant sequence homology.


Source

Registry

References

  • Pasotti L, Quattrocelli M, Galli D et al. (2011) Multiplexing and demultiplexing logic functions for computing signal processing tasks in synthetic biology. Biotechnol. J. 6(7):784-95.
  • Sleight SC, Bartley BA, Lieviant JA et al. (2010) Designing and engineering evolutionary robust genetic circuits. J. Biol. Eng. 4:12.