Plasmid_Backbone

Part:BBa_K516999:Experience

Designed by: Nicolo' Politi, Susanna Zucca, Tommaso Goggia, Paolo Magni   Group: iGEM11_UNIPV-Pavia   (2011-09-15)
Revision as of 19:26, 23 September 2011 by Nickpv (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K516999

User Reviews

UNIQ31f881ddddc872a0-partinfo-00000000-QINU UNIQ31f881ddddc872a0-partinfo-00000001-QINU


UNIQ31f881ddddc872a0-partinfo-00000002-QINU

•••••

UNIPV-Pavia iGEM 2011

This vector was designed and realized in order to facilitate the cloning of coding sequences downstream of the strong promoter pTet. This vector was assembled by ligating S-P excided mRFP coding sequence from BBa_J61002 and ligating it in BBa_R0040 cut with S and P. Thus, the resulting vector contains mRFP between S and P. pTet can be easily excided (E-P) and moved in the desired vector (E-P) and then the desired coding sequence can be easily assembled by digesting S-P the vector and X-P the coding sequence, thus obtaining a final part that is standard10-compatible.

UNIQ31f881ddddc872a0-partinfo-00000005-QINU