Coding
T7Amb

Part:BBa_K606032

Designed by: Axel Séguret   Group: iGEM11_Paris_Bettencourt   (2011-09-18)
Revision as of 18:28, 23 September 2011 by Kwarteng2 (Talk | contribs)

T7 RNA polymerase with tyrosine amber codons

T7 RNA polymerase with two tyrosines mutated to an amber (stop) codons.

Usage and Biology

The T7 RNA polymerases have two stops codons at the begening of its sequence. The protein cannot be expressed unless a tRNA amber supressor is expressed in the cell. (for instance K606034 for B. subtilis). This strategy can be use to create an and gate. If an amplified signal is required, K606018 or K606035 can be used for reporting.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
None