Part:BBa_B0031:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

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••••• Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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••••• UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5. See Experience page of BBa_B0034for more details. Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter: RBS effpLux effpTet effJ23101 Declared efficiency B0031 0.40 1.6814 2.45 0,6 B0031 0.01 ND 0.04 0,07 B0032 0.19 0.4193 0.40 0,3 B0034 1 1 1 1 Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI: RBS effmRFP effAiiA effLuxI Declared efficiency B0031 1.72 0.96 0.45 0,6 B0031 0.03 0.78 0.028 0,07 B0032 0.37 0.51 N.D. 0.3 B0034 1 1 1 1

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