Part:BBa_B0030:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQ6fc33c3b3c16cc4f-partinfo-00000000-QINU

••••• Antiquity

This review comes from the old result system and indicates that this part worked in some test.

••• Aberdeen_Scotland 2009

The miniprep, single and double digests all worked. However we did not use this part for further cloning.

UNIQ6fc33c3b3c16cc4f-partinfo-00000003-QINU

UNIQ6fc33c3b3c16cc4f-partinfo-00000004-QINU

••••• UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5. See Experience page of BBa_B0034for more details. Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter: RBS effpLux effpTet effJ23101 Declared efficiency B0030 0.40 1.6814 2.45 0,6 B0031 0.01 ND 0.04 0,07 B0032 0.19 0.4193 0.40 0,3 B0034 1 1 1 1 Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI: RBS effmRFP effAiiA effLuxI Declared efficiency B0030 1.72 0.96 0.45 0,6 B0031 0.03 0.78 0.028 0,07 B0032 0.37 0.51 N.D. 0.3 B0034 1 1 1 1

UNIQ6fc33c3b3c16cc4f-partinfo-00000007-QINU