Part:BBa_C0061:Experience

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User Reviews

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Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

••••• wmholtz

Using this part, I have successfully constructed and tested a quorum sensing circuit in E. coli.

••••• Youri

This part was used as a subpart in K546005, K546546 and found to be functional.

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••••• UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5. The LuxI enzyme activity has been characterized under the regulation of ptet promoter, assaying its enzymatic activity, with these parts: BBa_K516210 (wiki name: E13 ) pTet-RBS30-LuxI BBa_K516211 (wiki name: E14 ) pTet-RBS31-LuxI BBa_K516212 (wiki name: E15 ) pTet-RBS32-LuxI BBa_K516214 (wiki name: E16 ) pTet-RBS34-LuxI Though these parts don't have a transcriptional terminator, they have been characterized in low copy plasmid pSB4C5, that contains the BBa_B0054 terminator. The four measurment parts ptet-RBSx-LuxI were quantitatively characetrized using the BBa_T9002 biosensor see modelling section. The HSL synthesis rate has been evaluated according to the model equations, properly adjusted. A significative HSL production was observed for BBa_K516210, BBa_K516211 and BBa_K516214, while no HSL production was observed for BBa_K516212. The parameters Vmax, kM,LuxI and αRBSx were estimated with a simultaneous fitting of the data collected as described in measurement section: Vmax kM,LuxI αB0030 αB0031 αB0032 αB0034 3.56*10-9 6.87*103 87 8.5 ND 252

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