Part:BBa_K590025

The PetroBrick: Strong Constitutive Expression of ADC and AAR in pSB1C3

This part encodes an Acyl-ACP Reductase (AAR (BBa_K590032)) and Aldehyde decarbonylase (ADC (BBa_K590031)) from Synechococcus elongatus PCC7942 behind a strong constituitive expression cassette, BBa_K314100 in the pSB1C3 vector. Transforming this part into any strain of E. coli will result in the production of alkanes, the primary component of diesel, without the need for any inducing agents. More information on the optimal conditions for alkane production as well as detailed protocols for growth, production, and analysis of alkanes can be found on the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team Wiki page].

Usage and Biology

When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.

GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for [http://2011.igem.org/Team:Washington/Alkanes/Results details])

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 470
  Illegal NheI site found at 493
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1254
  Illegal XhoI site found at 2366
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 126
  
• 1000
  COMPATIBLE WITH RFC[1000]