Coding
mamQRB

Part:BBa_K590007

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 06:10, 22 September 2011 by Saushun (Talk | contribs) (→‎Usage and Biology)

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mamQRB, from Magnetospirillum magneticum

This part includes gene mamQ, mamR, and mamB, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

They are gene AMB0972, AMB0973, and AMB0974 respectively. mamQ was reported to be important for formation/maintenance of magnetosome membranes; mam R plays a role in controlling both particle number and size of magnetite cyrstals; mamB has indirect role in magnetosome membrane invagination and biomineralization; magnetosome compartment formation.

Below is the gel image of mamQRB (2029bp):

1kb Ladder (left), mamQRB (right)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 990
    Illegal BglII site found at 1005
    Illegal BglII site found at 1574
    Illegal XhoI site found at 1067
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1813
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 1365
    Illegal NgoMIV site found at 1973
    Illegal AgeI site found at 1631
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/amamQRB, from Magnetospirillum magneticum