Coding
mamJ

Part:BBa_K590002:Experience

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 05:47, 22 September 2011 by Saushun (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K590002

User Reviews

The part is contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Background Magnetosome toolkit]. It served as the template to amplify the Gibson Cloning friendly version of J to make Part:BBa_K90017, and Part: BBa_K590020. The repetitive region in the middle of the mamJ sequence made it tricky to verify, we turned to the Komeili lab help, and we were suggested to use CGGTGTCCGTCTCCGA as the primer to verify the middle part of J sequence.


We also tried to fuse mamJ with fluorescent proteins on 1A3 backbone, although we could not verify the correct sequence, we observed some interesting expressions at the interesting images below.

Igem2011 SfGFP-J-1A3-100iptg-01.jpg
Igem2011 RFP-J-1A3-100iptg-02.jpg


UNIQaa513c14db75074a-partinfo-00000000-QINU UNIQaa513c14db75074a-partinfo-00000001-QINU