Part:BBa_K606046:Experience

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Characterization of the sporulation induced by the expression of KinA

We suceeded in recovering the KinA gene from the non biobricked plasmid synthetized de novo by the 2009 Newcastle team, and cloned it into a standard biobrick plasmid, pSB1C3. Then we cloned this gene in front of the pVeg-SpovG (K143051) promoter + RBS. These two constructs had bees sended to the registry into pSB1C3.

We used a strain holding a KinA gene under the control of a hyperspank promoter. Growing the cell into a synthetic minimal media for hours, we saw the cell starting soprulating under the microscope after 1h30. Here are the images from the characterization.

Ref: Single,chemically defined sporulation medium for bacillus subtilis: growth,sporulation,and extracellular protease production. James H.HAGEMAN et al

We suceeded in recovering the KinA gene from the non biobricked plasmid synthetized de novo by the 2009 Newcastle team, and cloned it into a standard biobrick plasmid, pSB1C3. Then we cloned this gene in front of the pVeg-SpovG (K143051) promoter + RBS. These two constructs had been sended to the registry into pSB1C3.

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