Part:BBa_K606036:Experience

{| border="1" class="wikitable" style="text-align: center;" |+T7 autoloop in E.coli at 37°C |- |[[File:T7 autoloop ctrl-25.jpg|450px|thumb|center|E.coli T7 autoloop without terminator at 37°C (trans image)]] |[[File:T7 autoloop ctrl-26.jpg|450px|thumb|center|E.coli T7 autoloop without terminator at 37°C (gfp image)]] |- |[[File:T7_loop_1.jpg|450px|thumb|center|E.coli T7 autoloop with terminator at 37°C (trans image)]] |[[File:T7 loop 1 gfp.jpg|450px|thumb|center|E.coli T7 autoloop with terminator at 37°C (gfp image)]] |- |[[File:T7 autoloop ctrl-10.jpg|450px|thumb|center|E.coli negative control at 37°C (trans image)]] |[[File:T7 autoloop ctrl-11.jpg|450px|thumb|center|E.coli negative control at 37°C (gfp image)]] |} These pictures show that the T7 GFP autoloop system is efficient since some cells are glowing with GFP fluorescence. Thus, we can conclude that the T7 polymerase is activated. Finally, we notice there is no difference between the GFP autoloop with and without terminator before the T7 promoter, we can interpret this due to this terminator is a B.subtilis terminator, moreover, we know that this E.coli strain has 4 terminators.