Part:BBa_K608102

CcaS, green light receptor

CcaS is a cyanobacteriochrome, which is known to chromatically regulate the expression of the phycobilisome linker gene (cpcG2) in Synechocystis (see references). It functions as the receptor for green light and is made up of a N-terminal transmembrane helix, a cyanobactreria specific GAF domain, two PAS domains and a C-terminal histidine kinase. To be fully functional CcaS has to bind the chromophore Phycocyanobilin (PCB) with its GAF-domain. The GAF domain in this system has the ligation motif Cys-Leu, instead of the usual plant GAF-domain with Cys-His

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1685
  Illegal SpeI site found at 1780
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1685
  Illegal NheI site found at 1056
  Illegal SpeI site found at 1780
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1685
  Illegal XhoI site found at 6
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1685
  Illegal SpeI site found at 1780
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1685
  Illegal SpeI site found at 1780
  Illegal AgeI site found at 1720
  
• 1000
  COMPATIBLE WITH RFC[1000]

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References

Yuu Hirose et al ( 2008 )"Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein." Proc Natl Acad Sci U S A. 105: 9528–9533.

Tabor, J. J. et al.(2010), " Multichromatic Control of Gene Expression in Escherichia coli", J. Mol. Biol. , doi:10.1016/j.jmb.2010.10.038