Regulatory

Part:BBa_K530003:Design

Designed by: Daniel Wolozny   Group: iGEM11_Johns_Hopkins   (2011-08-04)
Revision as of 03:32, 22 September 2011 by Danielw9 (Talk | contribs) (→‎Design Notes)

KRE9 Yeast Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promoter was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the promoter. PCR and tube contents This PCR was done with the use of Herculase Enzyme from Agilent.

Source

http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=S000003710

References