Part:BBa_K515104

J23100 promoter - Antiholin

A composite part between J23100 and anti-holin (BBa_K515004). The promoter has been chosen in order to comply with our Gene Guard device specifications. It is under the influence of a strong promoter due to the lower copy number of this gene (will be integrated into the genome) in comparison to that of the Holin gene which will be in a high copy number plasmid within our system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 276
  

This BioBrick has been sequence verified. It has also been tested for protein expression. A nice clear band of the appropriate size can be seen at the bottom of the gel indicating a strong over-expression of a small protein of the appropriate size.

Figure 1: Protein gel showing an over-expression band (higher in intensity) of a small protein. Lane 1 and 2 are control and lane 3 and 4 are the anti-holin expressing cells.(Data by Imperial College London iGEM team 2011).