Coding

Part:BBa_K613014:Design

Designed by: Lilia Salimova   Group: iGEM11_EPF-Lausanne   (2011-09-20)
Revision as of 01:24, 22 September 2011 by Nguenat (Talk | contribs) (→‎Design Notes)

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TetR V36FW43S mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutation was introduced by PCR-induced mutagenesis, following data about the V36F mutation in based in [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007].

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36FW43S mutation by site-directed mutagenesis. The V36F mutation had already been described by Krueger et al (2007).

References