Intermediate

Part:BBa_K568005:Design

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-14)
Revision as of 00:13, 22 September 2011 by FabianFroehlich (Talk | contribs) (→‎Design Notes)

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Intermediate optogenetical AND-Gate without T7ptag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 3817
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3820


Design Notes

This part was constructed in order to add T7ptag (BBa_K228000) at 3' site of intermediate construct in a next step.

Source

The red light sensor (BBa_K568000) was added to an intermediated part of the optogenetic AND gate (BBa_K568006).

References