Generator

Part:BBa_K638401

Designed by: Matthew Jones   Group: iGEM11_Cambridge   (2011-09-19)
Revision as of 23:23, 21 September 2011 by Cat (Talk | contribs)

Arabinose inducible TorA Export Tagged Reflectin A1 Poly His generator

This construct is a Reflectin A1 protein with a TorA signal peptide fused to the N terminus, which has been shown to allow export of GFP via the twin arginine pathway (see information on Part:BBa_K638402). In addition a poly his tag has been added to the C terminus and a pBAD promoter will control expression of the fusion protein on induction with arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1369
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
n/aArabinose inducible TorA Export Tagged Reflectin A1 Poly His generator